Species: All species
Specimen: Whole blood
Container: EDTA (purple top)
Mix blood and anticoagulant gently and as soon as possible to prevent clotting. Make and air dry a blood smear as soon as possible to prevent artefactual changes occurring in the RBCs and WBCs. Do not refrigerate smears. Keep smears away from flies.
Special handling/shipping requirements: Make a smear as soon as possible after collection of blood as some blood parasites/organisms may fall off the RBCs.
General information about when this test is indicated: When anaemia is detected or suspected.
Organisms present in New Zealand include:
- Cats: Mycoplasma haemofelis (formerly Haemobartonella felis), Candidatus Mycoplasma haemominutum, Candidatus Mycoplasma turicensis
- Cattle: Theileria orientalis – Ikeda, Chitose, Buffeli, Mycoplasma wenyonii (formerly Eperythrozoan wenyonii), Candidatus Mycoplasma haemobos
- Sheep: Mycoplasma ovis (formerly Eperythrozoan ovis)
- Alpacas – Candidatus Mycoplasma haemolamae
- Dogs – Mycoplasma haemocanis has been reported but presence was not confirmed by PCR
Comparison with other related tests: See PCR section for specific tests. A PCR test is available for feline haemotropic mycoplasma confirmation if required. This requires a fresh EDTA blood sample or a sample that has not already been used for testing via an analyser, due to the very small chance that there may be transfer of organisms from a prior infected sample.